Production of Bacillus Subtilis as Biological Fertilizer by Biotransformation of Corn Steep Liquor

Corn steep liquor is a kind of intractable organic waste liquidproduced during the processing corn starch. But it rich in nutrients,soluble protein, and amino acids could be used as nitrogen source inthe fermentation industry. Many starch enterprises cannot directlyutilize choose to discharge directly, thus causingenvironmental pollution. Studies have shown that high ammonia cornsteep can promote increase microbial biomass. B. subtilisis dominant strain flora liquor, classicstrain research industrial production biological bacterialfertilizer. In this study, subtilis 3301, which issuitable for high-density cultivation was screened first.Then optimal parameters, like concentration,pH, inoculation amount bacteria were analyzed by three-factor andthree-level Response Surface (RSM) analysis experiment. The highest celldensity obtained when concentration 145 g·L-1, pH 7 inoculationamount 8. Then dynamic changes cultured cornpulp beef extract peptone medium (NB) compared under response surfaceoptimization conditions. results showed subtiliscultured pulp significantlyhigher than NB medium. Finally, effect fed-batch glucoseon culture investigated andthe feeding with glucose, number viablebacteria reach 1.75 × 1010 CFUmL-1, issignificantly higher viable without feedingglucose. All above show optimized using cheapcorn-steep shows good level. provide atheoretical reference further improving biomass bacterialfertilizer at same time practical theindustrial bacterial fertilizer reduce cost ofraw materials, alleviate pollution environmentand development crop